Lab Protocols (pdf files)
Cell Culture
Agar plates
Preparation of competent cells
Quantification
Plasmids / larger nucleic acids
Proteins via A
280
SH groups in proteins
Amplification, Manipulation and Cloning
Oligo Extinction Coefficient Calculator
Standard PCR
Purification of DNA with agarose gels
Digestion of DNA with RE's and dephosphorylation of vector
Ligation with Rapid Ligation Kit
Transformation into chemically competent cells
Colony PCR: Screening for inserts
Introducing point mutations
PAGE
Coomassie blue stain
Monobromobimane labeling of thiol groups
Silver stain
Tris-Tricine gel electrophoresis
Buffers and Stock Solutions
6xAgarose gel loading buffer
Antibiotics, Stock solutions
25% APS solution
50xTAE buffer
TBF buffers fot chemically competent cells
Tris-Tricine gel electrophoresis
Data Fitting
Part I: Derivation of pK
a
-equations for fitting of absorption data, etc.
List of pK
a
-equations for fitting of absorption data, etc. (doc file)
List of pK
a
-equations for fitting of absorption data, etc. (pdf file)
Part II: Derivation of equation for two independent pK
a
values
Instrumentation
UV-Vis Calibration